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rh interleukin-3  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rh interleukin-3
    Rh Interleukin 3, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rh+interleukin-3/pmc10559794-62-37-48?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    rh interleukin-3 - by Bioz Stars, 2026-08
    90/100 stars

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    Long-term cryopreserved CB has similar ex vivo growth properties compared with fresh and recently cryopreserved CB (A–H) We plated 5 × 10 4 CD34 + cells were plated in RPMI1640 media with SCF, TPO, and FLT3L grown for 4 days. (A–F) Immunophenotypic HSC/HPC numbers at day 0 and day 4. (G–H) CFU numbers from input day 0 or day 4 expanded CD34 + cells plated with fetal bovine serum <t>and</t> <t>rhEPO/SCF/IL-3/GM-CSF</t> in methylcellulose media. (I) Summary table of expansion expressed as day 4/day 0 ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. ns, not significant. n = 3 biological replicates for 27yoCB and 3yoCB, n = 4 biological replicates for frCB. Expansion assays were plated without technical replication. All CFU assays were plated and counted in technical triplicates. Error bars indicate standard deviation. Colors of points: 27yoCB, orange = CB239; green = CB397; blue = CB410; 3yoCB, purple = CB415; red = CB609; pink = CB320.
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    Long-term cryopreserved CB has similar ex vivo growth properties compared with fresh and recently cryopreserved CB (A–H) We plated 5 × 10 4 CD34 + cells were plated in RPMI1640 media with SCF, TPO, and FLT3L grown for 4 days. (A–F) Immunophenotypic HSC/HPC numbers at day 0 and day 4. (G–H) CFU numbers from input day 0 or day 4 expanded CD34 + cells plated with fetal bovine serum <t>and</t> <t>rhEPO/SCF/IL-3/GM-CSF</t> in methylcellulose media. (I) Summary table of expansion expressed as day 4/day 0 ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. ns, not significant. n = 3 biological replicates for 27yoCB and 3yoCB, n = 4 biological replicates for frCB. Expansion assays were plated without technical replication. All CFU assays were plated and counted in technical triplicates. Error bars indicate standard deviation. Colors of points: 27yoCB, orange = CB239; green = CB397; blue = CB410; 3yoCB, purple = CB415; red = CB609; pink = CB320.
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    Long-term cryopreserved CB has similar ex vivo growth properties compared with fresh and recently cryopreserved CB (A–H) We plated 5 × 10 4 CD34 + cells were plated in RPMI1640 media with SCF, TPO, and FLT3L grown for 4 days. (A–F) Immunophenotypic HSC/HPC numbers at day 0 and day 4. (G–H) CFU numbers from input day 0 or day 4 expanded CD34 + cells plated with fetal bovine serum and rhEPO/SCF/IL-3/GM-CSF in methylcellulose media. (I) Summary table of expansion expressed as day 4/day 0 ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. ns, not significant. n = 3 biological replicates for 27yoCB and 3yoCB, n = 4 biological replicates for frCB. Expansion assays were plated without technical replication. All CFU assays were plated and counted in technical triplicates. Error bars indicate standard deviation. Colors of points: 27yoCB, orange = CB239; green = CB397; blue = CB410; 3yoCB, purple = CB415; red = CB609; pink = CB320.

    Journal: Cell Reports Medicine

    Article Title: Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood

    doi: 10.1016/j.xcrm.2023.101259

    Figure Lengend Snippet: Long-term cryopreserved CB has similar ex vivo growth properties compared with fresh and recently cryopreserved CB (A–H) We plated 5 × 10 4 CD34 + cells were plated in RPMI1640 media with SCF, TPO, and FLT3L grown for 4 days. (A–F) Immunophenotypic HSC/HPC numbers at day 0 and day 4. (G–H) CFU numbers from input day 0 or day 4 expanded CD34 + cells plated with fetal bovine serum and rhEPO/SCF/IL-3/GM-CSF in methylcellulose media. (I) Summary table of expansion expressed as day 4/day 0 ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. ns, not significant. n = 3 biological replicates for 27yoCB and 3yoCB, n = 4 biological replicates for frCB. Expansion assays were plated without technical replication. All CFU assays were plated and counted in technical triplicates. Error bars indicate standard deviation. Colors of points: 27yoCB, orange = CB239; green = CB397; blue = CB410; 3yoCB, purple = CB415; red = CB609; pink = CB320.

    Article Snippet: In short, CFU-GM, BFU-E, and CFU-GEMM colonies were derived by plating 250, 500, and 2000 CD34 + cells in 1% methylcellulose/Iscove’s Modified Dulbeccos Medium (IMDM) with 30% FBS (Corning, prescreened), 2 U/mL recombinant human (rh) Epogen (EPO) (Amgen), 10ng rh Interleukin-3 (IL-3) (R&D Systems), and with or without 50ng rh Stem cell factor (SCF) (R&D Systems), and 10ng rh Granulocyte/macrophage colony stimulating factor (GM-CSF) (R&D Systems).

    Techniques: Ex Vivo, Standard Deviation